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KMID : 0545120020120060986
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 6 p.986 ~ p.988
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Simple Purification of Shiga Toxin B Chain from Recombinant Escherichia coli
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OH, YOUNG PHIL
JEONG, SEONG TAE/KIM, DAE-WEON/KIM, EL-CHAE/YOON, KI-HONG
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Abstract
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A plasma expression vector of pEStx1 encoding a mature form of the B chain of the Shiga toxin was constructed without a signal peptide under the control of an inducible T7 promoter. The encoded protein was purified to 90% by simple heat treatment, and then further purified to 95% by Phenyl-Sepharose and DEAE- Sepharose chromatographies, all in a single day. Accordingly, this expression system and heat treatment could facilitate the rapid purification of gram-scale amount of the Shiga toxin B subunit from recombinant Esherichia coli cells.
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KEYWORD
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